NucleoCounter® NC-250™

Brand:

  • Chemometec

The NucleoCounter® NC-250™ is an automated cell analyzer, which can characterize cell properties using fluorescence imaging. The NC-250™ offers a wide range of optimized protocols to meet the needs of all mammalian cell types grown under different conditions.

Through unique assays, cell count can be performed even on cells growing on microcarriers and in sphere cultures. This benchtop image cytometer ensures high-precision and does not require calibration.

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Advanced Image Cytometer

NucleoCounter® NC-250™ Automated Cell Analyzer Features

  • Fast, high-precision cell counts
  • Analyzes total cell count and viability for 8 samples in 3 minutes
  • Easy to operate
  • Excellent data reproducibility
  • No calibration required
  • 21 CFR Part 11/GMP-ready

Description

The NucleoCounter® NC-250™ is an automated cell analyzer, which can characterize cell properties using fluorescence imaging. The NC-250™ offers a wide range of optimized protocols to meet the needs of all mammalian cell types grown under different conditions.

Through unique assays, cell count can be performed even on cells growing on microcarriers and in sphere cultures. This benchtop image cytometer ensures high-precision and does not require calibration.

With a combination of simplicity and innovation, counting and viability determination of mammalian cells has never been easier. Simply mix a small volume of cell suspension with fluorescent dyes, load, and run. Within three minutes you will have eight highly precise viability and cell count analyses at hand.

For cells with low concentration, or to achieve even higher precision, users can take advantage of the NC-Slide A2™ with a 30 µl volume capacity.

The NucleoCounter® NC-250™ automated cell analyzer performs high-speed cell count and viability determination and offers a unique 5-minute cell cycle assay and a 1-minute apoptosis assay for up to eight samples at the time.

The intuitive software has unlimited licenses and produces on-screen data analysis and automated PDF reports.

Specifications

Application type(s)

Cell count & viability, Cell cycle, Vitality.

Cell type(s)

Mammalian, yeast, insect, avian

Software

NucleoView™

Consumable(s)

NC-Slide A2™ and NC-Slide A8™

Capacity

Variable, depending on consumable and assay type

Analysis time

Variable, depending on consumable and assay type

Analysis volume

0.8 – 16 μl sample (NC-Slide A2™, NC-Slide A8™)

Optimal range

5×10^4 to 5×10^6 cells/ml

Staining dye(s)

Acridine orange (AO), DAPI, VitaBright-48™, Propidium iodide (PI), User-defined

Loading volume

30 μl (NC-Slide A2™), 10 μl (NC-Slide A8™)

Excitation

Two LED light sources with peaks at 365 nm and 500 nm + one dark field white LED

Emission (nm)

A single dual-band emission filter: 410 – 450 nm and 520 – 595 nm

Optics

Lens with ×1.75 magnification, 1/2.5” CMOS camera

Data output

Images, tables, histograms, scatter plots

Data export formats

.cm, .csv, .pdf, .cmpp, .bmp, .tiff, .acs, .fcs

Product number

970-0251

Size

19 × 29 × 32 cm (8 × 12 × 13”) (W × H × D)

Weight

9 kg (20 lbs)

Supply Voltage

24VDC (100-240V~ 50-60Hz)