NucleoCounter® NC-3000™

Brand:

  • Chemometec

The NucleoCounter® NC-3000™ advanced image cytometer utilizes fluorescence imaging to characterize cell properties. The instrument performs high-speed plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay, a selection of apoptosis assays and the standard cell count and viability assay.

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Advanced Image Cytometer

  • A variety of fixed and build-your-own assays
  • High-speed cell counting and advanced data analysis
  • No warm-up or calibration required
  • No liquid waste produced
  • 21 CFR Part 11/GMP-ready software

Description

The NucleoCounter® NC-3000™ advanced image cytometer utilizes fluorescence imaging to characterize cell properties. The instrument performs high-speed plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay, a selection of apoptosis assays and the standard cell count and viability assay.

With the FlexiCyte™ license, you can create protocols with options for light source, emission filters and other detailed settings. The accompanying NucleoView™ software is ideal for intuitive data analysis and has unlimited licenses. You can view data in plot graphs and interlinked images, which can be saved for later on-demand analysis.

This advanced image cytometer ensures flow-like analysis with high reproducibility, operates with low sample volumes, and is calibration-free.

Used with the disposable Via1-Cassette™, this closed cell counting system helps you stay protected when handling samples. No pipettes, no exposure to (toxic) reagents, and minimal clean-up make the NucleoCounter® NC-3000™ a safe option.

Specifications

Application type(s)

Fixed assays: Cell count & viability, Cell cycle, Mitochondrial potential, Vitality, Caspase, Annexin V, Transfection efficiency, DNA fragmentation assays. User-adapted protocols with the Flexicyte™ module

Cell type(s)

Mammalian, yeast, insect, avian

Software

NucleoView™

Consumable(s)

Via1-Cassette™, NC-Slide A2™ and NC-Slide A8™

Capacity

Variable, depending on consumable and assay type

Analysis time

Variable, depending on consumable and assay type

Analysis volume

3.2 μl sample (Via1-Cassette™), 0.8 – 16 μl sample (NC-Slide A2™, NC-Slide A8™)

Optimal range

5×104 to 5×106 cells/ml (for counting)

Staining dye(s)

Hoechst 33342, JC-1, VitaBright-43™, VitaBright-48™, Acridine orange (AO), DAPI, Propidium iodide (PI), User-defined

Loading volume

60 μl (Via1-Cassette™), 30 μl (NC-Slide A2™), 10 μl (NC-Slide A8™)

Excitation

Seven LED light sources with peaks at 365 nm, 405 nm, 475 nm, 530 nm, 630 nm + 2 white LEDs

Emission

Nine interchangeable emission filters: 430/20, 470/55, 475/15,
530/15, 560/35, 580/25, 675/75, 630 LP, 740/60

Optics

Lens with x2 magnification, 1/2″ CCD camera with 1392×1040 pixels

Data output

Images, tables, histograms, scatter plots

Data export formats

.cm, .csv, .pdf, .cmpp, .bmp, .tiff, .acs, .fcs

Product number

970-3002 (Fixed Analytical Assays); 970-3003 (FlexiCyte™)

Size

29 x 29 x 31 cm (W x H x D)

Weight

14 kg (30.9 lb)

Supply voltage

24VDC (100-240V ~ 50-60Hz)