The PI-Cassette™ is a specialized cell sampling and staining device for viability and cell count applications with the NucleoCounter® NC-3000™ instrument. It contains immobilized propidium iodide (PI) in its interior flow channels.

Loading approximately 60 µl of cell sample into the cassette dissolves the PI and mixes it with the sample to stain the nuclei of dead cells.

Approximately 60 µl of cell sample is loaded into the cassette, dissolving the PI and mixing to stain the nuclei of dead cells. PI-Cassettes™ can be used to perform viability and cell counts in conjunction with Solution 10 Lysis Buffer and Reagent Y100, respectively, on mammalian and yeast cells. Cells are subsequently analyzed on the NucleoCounter® NC-3000™.